Optimization of enzyme‐assisted extraction of ferulic acid from sweet corn cob by response surface methodology

Lau, T, Harbourne, N and Oruña‐Concha, M.J (2020) 'Optimization of enzyme‐assisted extraction of ferulic acid from sweet corn cob by response surface methodology.' Journal of the Science of Food and Agriculture, 100 (4). pp. 1479-1485. ISSN 0022-5142

Abstract

BACKGROUND Sweet corn cob (SCC), an agricultural by-product of the corn-processing industry, contains more than 80% insoluble bound ferulic acid (FA). Extraction of these bound phenolics can be achieved through chemical or enzymatic hydrolysis; however, the shift towards greener chemistry has raised awareness about the use of enzymatic hydrolysis. In the present study, the ability of ferulic acid esterase (FAE) and xylanase (XY) to catalyze the hydrolysis of FA from SCC was investigated. Response surface methodology (RSM), based on a five-level, four-factor central composite rotatable design (CCRD), was used to establish the optimum conditions for enzymatic hydrolysis of FA from SCC. Sweet corn cob was treated with a combination of FAE and XY at various concentrations (FAE: 0.00 to 0.04 U/g; XY: 0.00 to 18 093.5 U/g), temperatures (45 to 65 °C), and pH levels (pH 4.5 to 6.5). RESULTS The optimum extraction conditions predicted by the model were: FAE concentration of 0.02 U/g, XY concentration of 3475.3 U/g, extraction pH of 4.5, and an extraction temperature of 45 °C. CONCLUSION Under these conditions, the experimental yield of FA was 1.69 ± 0.02 g kg−1 of SCC, which is in agreement with the value predicted by the model.

Item Type:	Article
Keywords:	sweet corn cob, response surface methodology, ferulic acid, ferulic acid esterase, xylanase
Divisions:	School of Sciences
Identification Number:	https://doi.org/10.1002/jsfa.10155
Date Deposited:	22 Sep 2023 15:04
Last Modified:	22 Sep 2023 15:04
URI / Page ID:	https://researchspace.bathspa.ac.uk/id/eprint/15721

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