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Visualization of the cytoskeleton within the secondary vascular system of hardwood species

Chaffey, N, Barnett, J.R and Barlow, P.W (1997) 'Visualization of the cytoskeleton within the secondary vascular system of hardwood species.' Journal of Microscopy, 187 (2). pp. 77-84. ISSN 1365-2818

Abstract

A single fixation technique has been devised to demonstrate localization of α-tubulin (for microtubules) and F-actin (for microfilaments) within the secondary vascular system of hardwood trees by indirect immunofluorescence microscopy using butyl-methylmethacrylate-embedded material. Application of this technique to problems of cytomorphogenesis during secondary growth and its versatility are demonstrated with the hardwood species Aesculus hippocastanum L., Salix viminalis L., S. burjatica Nazarov × S. viminalis L., Hedera helix L., Acer platanoides L., Platanus sp., Quercus ilex L. and Liriodendron tulipifera L., and in the softwood Pinus pinea L. The methods employed have considerable scope for advancing knowledge of the role of the cytoskeleton in differentiation within the secondary vascular system of woody species.

Item Type: Article
Keywords: α-Tubulin;cytoskeleton; F-actin;hardwood trees; indirect immunofluorescence microscopy; microfilaments; microtubules; removable embedment; secondary vascular tissue; vascular cambium
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany
Divisions: College of Liberal Arts
Date Deposited: 20 Jul 2015 11:59
Last Modified: 29 Apr 2016 14:12
URI: http://researchspace.bathspa.ac.uk/id/eprint/6347
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