Chaffey, N (1985) 'Structure and function in the grass ligule: mitochondria and microbodies in the ligule of Lolium temulentum L., an ultrastructural and cytochemical study.' Journal of Experimental Botany, 36 (6). pp. 1001-1007. ISSN 0022-0957

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The ultrastructure and distribution of the mitochondria and microbodies within the tissues of the ligule of mature leaves of Lolium temulentum L. are described. Staining of the mitochondria and microbodies with diaminobenzidine (DAB) was observed. Mitochondrial staining was totally inhibited by potassium cyanide and sodium azide but not by aminotriazole, and did not occur in the presence of added hydrogen peroxide; it was concluded that cytochrome c oxidase (EC was responsible. In the microbodies staining was reduced in the absence of added hydrogen peroxide and eliminated in the presence of aminotriazole; it was concluded that catalase (EC was responsible. Presence of succinic dehydrogenase (EC activity was demonstrated in the mitochondria using the copper-ferricyanide reaction; presence of sodium malonate and absence of sodium succinate greatly reduced mitochondrial deposition of reaction product. The results are discussed in relation to possible functions of these organelles in the ligule.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany
Divisions: College of Liberal Arts
Date Deposited: 20 Jul 2015 12:31
Last Modified: 29 Apr 2016 14:12
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